UV/Vis spectrophotometers are usually dual-beam spectrophotometers where the first channel contains the sample and the second channel holds the control for correction. A spectrophotometer has two fundamental parts: a source of radiant light and a monochromator. Monochromator is placed between the light source and the sample which disperses the radiant energy into a spectrum. A slit is also fixed which selects a narrow portion of the spectrum. The standard cell or cuvette is placed in a light-tight-unit. The incident light strikes the standard cell and emergent light passes into a photocell. The photocell changes the emerging light energy into measureable electrical energy.

Applications

• Qualitative analysis and quantitative determination of UV/Vis active analytes, namely, organic compounds, biological macromolecules, etc.
• Drug identification and nucleic acid purity checks and quantitation, to quality control in the beverage industry and chemical research, etc.